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[The determination of the genotype of natural reassortant influenza A viruses according to the core protein genes by the methods of competitive dot hybridization and sequencing].

Identifieur interne : 002D83 ( Main/Exploration ); précédent : 002D82; suivant : 002D84

[The determination of the genotype of natural reassortant influenza A viruses according to the core protein genes by the methods of competitive dot hybridization and sequencing].

Auteurs : E B Grinbaum ; F N Zolotarev ; N A Petrov ; O M Litvinova ; I B Konovalenko ; T Ia Luzianina ; D B Golubev

Source :

RBID : pubmed:1290222

Descripteurs français

English descriptors

Abstract

Simultaneous circulation of different subtypes of influenza A viruses provides conditions for reassortant strains formation. A comparative investigation of genome of 47 influenza A virus strains (H1N1, H2N2, and H3N2) was carried out by competitive dot hybridization technique and sequence analysis of some of cDNA-copies of the virus genes. All the genes of 43 strains encoding nonglycolysed proteins corresponded to the serum subtype of surface glycoproteins. The reassortant pattern of genome for some genes of core proteins was revealed in 4 viruses. All the dot hybridization data were completely confirmed by sequence analysis of the genes.

PubMed: 1290222


Affiliations:


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Le document en format XML

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<name sortKey="Petrov, N A" sort="Petrov, N A" uniqKey="Petrov N" first="N A" last="Petrov">N A Petrov</name>
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<name sortKey="Litvinova, O M" sort="Litvinova, O M" uniqKey="Litvinova O" first="O M" last="Litvinova">O M Litvinova</name>
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<term>Antigens, Viral (genetics)</term>
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<term>DNA, Viral (genetics)</term>
<term>Genotype</term>
<term>Influenza A virus (classification)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (immunology)</term>
<term>Nucleic Acid Hybridization (methods)</term>
<term>Plasmids (genetics)</term>
<term>Reassortant Viruses (classification)</term>
<term>Reassortant Viruses (genetics)</term>
<term>Reassortant Viruses (immunology)</term>
<term>Sequence Analysis, DNA (methods)</term>
<term>Serotyping</term>
<term>Viral Core Proteins (genetics)</term>
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<term>ADN (génétique)</term>
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<term>Antigènes viraux (génétique)</term>
<term>Génotype</term>
<term>Hybridation d'acides nucléiques ()</term>
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<term>Viral Core Proteins</term>
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<term>Influenza A virus</term>
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<term>Influenza A virus</term>
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<term>Reassortant Viruses</term>
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<term>Nucleic Acid Hybridization</term>
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<term>Analyse de séquence d'ADN</term>
<term>Génotype</term>
<term>Hybridation d'acides nucléiques</term>
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<div type="abstract" xml:lang="en">Simultaneous circulation of different subtypes of influenza A viruses provides conditions for reassortant strains formation. A comparative investigation of genome of 47 influenza A virus strains (H1N1, H2N2, and H3N2) was carried out by competitive dot hybridization technique and sequence analysis of some of cDNA-copies of the virus genes. All the genes of 43 strains encoding nonglycolysed proteins corresponded to the serum subtype of surface glycoproteins. The reassortant pattern of genome for some genes of core proteins was revealed in 4 viruses. All the dot hybridization data were completely confirmed by sequence analysis of the genes.</div>
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